In order for the embryo to implant in the lining of the uterus, it must undergo the necessary division where it forms a multi-cell embryo with a fluid cavity (blastocyst). The blastocyst is coated with a gel-like material called ‘zona’ or ‘zona pellucida’. To implant a blastocyte in the uterus, its cells must be removed from the uterus. This process is known as hatching.
This defect of the blastocyst is often weakened by IVF or ICSI. This happens when the hatching of the supporting egg is useful. It is a laboratory procedure where implantation is done by weakening the embryo chemically or mechanically to facilitate implantation. In 1990, Dr. at Cornell University in New York. The technique was first developed by Jacques Cohen, and has since been adopted by many IVF clinics around the world.
Who should consider assisted hatching?
Age factor; Especially when the female spouse is older than 37 years
Egg quantity and quality; When a woman’s day 3 levels of follicle stimulating hormone (FSH) are elevated, or with low antral follicular count or low AMH levels
Fetal quality factor; Poor quality of fetus with slow rate of cell division or excessive fragmentation
Zona factor; When the fetus has a gallbladder zone pellucida or a thick outer shell
Previous IVF failure; Women who have failed one or more IVF cycles
Egg hatching with IVF
One of the most frustrating aspects of IVF for patients and professionals, is the failure, especially for couples who have tried ART procedures too often. Assisted hatching helps couples cope with difficulties that persist over time. After observing the high rate of fetal transplantation with a thin ZP, it was speculated that creating a small defect in the ZP would eventually increase the likelihood of fetal miscarriage.
This has been a blessing in disguise for women who have suffered multiple shocks in the IVF cycle due to shell weakness of the same GZ zone. In addition, the assisted hatched embryo is implanted a day earlier which gives more chance for consent to implantation, especially in cases where the endometrium is advanced due to ovarian stimulation. Despite this additional laboratory manipulation, the risk of damage to the fetus during the process of micromanipulation and transfer is low, with a slight increase in identical twins. The size of the defect in this process, however, is important and can lead to unintended consequences.
Types of hatching from assisted eggs
Four types of assisted hatching techniques are currently being studied by ART experts around the world.
Partial zone dissection, also known as mechanical assisted hatching, partial zone dissection involves keeping the fetus in position by briefly sucking softly. The micro-needle passes through the ZP in the largest perivatlin and advanced tactile pathway. After making a cross-shaped incision, the fetus is freed from the pipe holding. Stored culture 7 returns up to embryo transfer.Acid Tyrode Assisted Hatching To blow the acid tyrode on the outer surface, a defect is created in the fetal area using a mouth-controlled delivery system. Embryos are often stored for frequent washing with acid and transferred back to standard culture media.
Laser hatching This method uses computer-controlled non-contact mode to pierce the zone. Laser-assisted hatching includes specialized software designed to enable fetal scaling with easy positioning, focusing, and simple laser alignment. The laser is also configured with low, medium and high three preset energy intensity which can deliver pulse with a single click of the mouse to equip the zone according to specific requirements.
A thin solution of Proves is used in this process to get out of the auxiliary egg by diluting the pronez of Z. jona pellucida. The embryos are transferred to G2 medium under oil in the Provas solution for initial stretching and softening. The purpose of this method is to dilute ZP without complete solutions. The results are then observed under a microscope, if necessary, served from the fetal post-test. These embryos are then transferred to fresh G2 medium and washed twice before placing in the incubator until transfer.
Help run ivf success rate
Auxiliary hatching by a properly trained specialist embryologist is important for success. The actual live birth rate from this ART procedure can vary from IVF clinic to clinic. As a woman’s fertility increases, the younger you are, the greater your chances of success. Research suggests that this process causes abnormalities rather than IVF without hatching.